Composite

Part:BBa_J147000:Design

Designed by: MAX CARBONELL BALLESTERO   Group: Sole Lab   (2015-08-03)


RFP inducible device regulated by LuxR and C6-HSL through the Lux promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 740
    Illegal AgeI site found at 852
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 101
    Illegal BsaI.rc site found at 2533


Design Notes

LuxR coding sequence and GFP coding sequence are in tandem, both placed after a Ribosome Binding Site sequence.


Source

This composite part has been created using the Biobrick assembly method with parts taken from the Spring 2010 iGEM distribution.

References